What makes this antibody ideal for IHC applications?

The AA98 clone's binding efficacy to human MCAM makes it ideal for IHC applications. Its use facilitates detailed examination of CD146, crucial for research into cellular adhesion phenomena, providing high-resolution insights into tumor-related studies.

Is the AA98 clone effective in inhibiting cancer cell markers?

Yes, the AA98 clone effectively inhibits markers linked to cancer cell adhesion and migration. Its application in flow cytometry and ELISA has provided valuable data on MCAM, contributing significant findings towards research in cellular dynamics and tumor inhibition mechanisms.

Mouse Anti-MCAM Recombinant Antibody (clone AA98)

CAT#: HPAB-0031-YJ

Mab AA98 is a recombinant mouse monoclonal antibody binds to human CD146, a marker on the tumor cells. It can significantly inhibit the expression of MDA-MB-231 cells interstitial cell marker Slug, upregulate epithelial cell marker E-cadherin and inhibit cell migration. It can also be used in the combined anti-tumor therapy with other antibodies.

Tested Data Gene Expression

ELISA

Figure 1 Mouse Anti-MCAM Recombinant Antibody (HPAB-0031-YJ) in ELISA

ELISA analysis of HPAB-0031-YJ was performed by coating with Human MCAM protein.
The secondary antibody: HRP-Anti-Mouse IgG (H+L)

Figure 2 Mouse Anti-MCAM Recombinant Antibody (HPAB-0031-YJ) in WB

WB analysis of HPAB-0031-YJ was performed with Human MCAM protein.

Lane1: Reducing antigen (0.1μg)
Lane2: Reducing antigen (0.3μg)
Lane3: Reducing antigen (0.6μg)

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Subcellular Location Figure 1 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2147/if_selected.jpg

Normal Tissue Figure 2 Soft tissue

Fibroblasts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8848/24112_B_2_7.jpg

Normal Tissue Figure 3 Cerebral cortex

Endothelial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear Neuronal cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8848/24112_B_9_5.jpg

Normal Tissue Figure 4 Colon

Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8848/24112_A_8_3.jpg

Normal Tissue Figure 5 Kidney

Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Distal tubules Staining: Medium Intensity: Strong Quantity: <25%

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8848/24112_A_7_5.jpg

Normal Tissue Figure 6 Testis

Elongated or late spermatids Staining: Medium Intensity: Moderate Quantity:>75% Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Peritubular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Sertoli cells Staining: Low Intensity: Weak Quantity:>75%

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8848/24112_A_4_6.jpg

RNA Expression Figure 7 RNA cell line category: Group enriched (HUVEC TERT2, TIME, WM-115)

Cell lines ordered by descending RNA expression order

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000076706-MCAM

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Specifications

Immunogen
HUVEC proliferation induced by the culture supernatant of hepatoma cells.

Host Species
Mouse

Type
Mouse IgG

Specificity
Human MCAM

Species Reactivity
Human

Clone
AA98

Applications
ELISA, FC, Inhib, IHC-Fr, WB, IP

Related Disease
Cancer, Tumor

Product Property

Purity
>95% as determined by SDS-PAGE and HPLC analysis

Concentration
Please refer to the vial label for the specific concentration.

Buffer
PBS

Preservative
No preservatives

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
The antibody AA98 has been reported in applications of Enzyme-linked Immunosorbent Assay, Flow Cytometry, Inhibition, Immunohistochemistry-Frozen, Western Blot, Immunoprecipitation. It's recommended that the optimal antibody concentration, dilution, incubition time etc. be carefully titrated in specific assays.
Inhibition:10 μg/mL anti-CD146 monoclonal antibody AA98 was added in the medium for inhibition.

Target

Alternative Names
CD146; MUC18; HEMCAM; METCAM; MelCAM; Melanoma Cell Adhesion Molecule; S-Endo 1 Endothelial-Associated Antigen; Melanoma-Associated Antigen MUC18; Cell Surface Glycoprotein P1H12; Melanoma-Associated Antigen A32; Gicerin; AA98

Gene ID
4162

UniProt ID
P43121

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Breast cancer biomarkers at key points during disease progression

Versatile Application

The AA98 clone serves multiple purposes in our research, especially for inhibiting tumor cell markers in cancer studies. Its reliable performance in ELISA and flow cytometry enriches our understanding of CD146 in cancer cell adhesion and migration studies, providing valuable insights.

Significant Inhibition

The anti-MCAM antibody has shown significant inhibition properties in our experiments, notably impacting cell migration processes. It has been effective in various applications, ensuring accurate analysis of MCAM's role in our cellular models, which is crucial for our research progress.

Q&As

What makes this antibody ideal for IHC applications?

A: The AA98 clone's binding efficacy to human MCAM makes it ideal for IHC applications. Its use facilitates detailed examination of CD146, crucial for research into cellular adhesion phenomena, providing high-resolution insights into tumor-related studies.
Is the AA98 clone effective in inhibiting cancer cell markers?

A: Yes, the AA98 clone effectively inhibits markers linked to cancer cell adhesion and migration. Its application in flow cytometry and ELISA has provided valuable data on MCAM, contributing significant findings towards research in cellular dynamics and tumor inhibition mechanisms.

View the frequently asked questions answered by Creative Biolabs Support.

Cite This Product

To accurately reference this product in your publication, please use the following citation information:

(Creative Biolabs Cat# HPAB-0031-YJ, RRID: AB_3111345)

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Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - HPAB-0031-YJ.pdf

MSDS

MSDS - HPAB-0031-YJ.pdf

COA

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