Mouse Anti-MCAM Recombinant Antibody (clone AA98)
CAT#: HPAB-0031-YJ
Mab AA98 is a recombinant mouse monoclonal antibody binds to human CD146, a marker on the tumor cells. It can significantly inhibit the expression of MDA-MB-231 cells interstitial cell marker Slug, upregulate epithelial cell marker E-cadherin and inhibit cell migration. It can also be used in the combined anti-tumor therapy with other antibodies.
Tested Data
Gene Expression
ELISA
Figure 1 Mouse Anti-MCAM Recombinant Antibody (HPAB-0031-YJ) in ELISA
ELISA analysis of HPAB-0031-YJ was performed by coating with Human MCAM protein.
The secondary antibody: HRP-Anti-Mouse IgG (H+L)
WB
Figure 2 Mouse Anti-MCAM Recombinant Antibody (HPAB-0031-YJ) in WB
WB analysis of HPAB-0031-YJ was performed with Human MCAM protein.
Lane1: Reducing antigen (0.1μg)
Lane2: Reducing antigen (0.3μg)
Lane3: Reducing antigen (0.6μg)
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Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2147/if_selected.jpg
Normal Tissue
Figure 2 Soft tissue
Fibroblasts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8848/24112_B_2_7.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear Neuronal cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8848/24112_B_9_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8848/24112_A_8_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Distal tubules Staining: Medium Intensity: Strong Quantity: <25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8848/24112_A_7_5.jpg
Normal Tissue
Figure 6 Testis
Elongated or late spermatids Staining: Medium Intensity: Moderate Quantity:>75% Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Peritubular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Sertoli cells Staining: Low Intensity: Weak Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8848/24112_A_4_6.jpg
RNA Expression
Figure 7 RNA cell line category: Group enriched (HUVEC TERT2, TIME, WM-115)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000076706-MCAM
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Specifications
- Immunogen
- HUVEC proliferation induced by the culture supernatant of hepatoma cells.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human MCAM
- Species Reactivity
- Human
- Clone
- AA98
- Applications
- ELISA, FC, Inhib, IHC-Fr, WB, IP
- Related Disease
- Cancer, Tumor
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody AA98 has been reported in applications of Enzyme-linked Immunosorbent Assay, Flow Cytometry, Inhibition, Immunohistochemistry-Frozen, Western Blot, Immunoprecipitation. It's recommended that the optimal antibody concentration, dilution, incubition time etc. be carefully titrated in specific assays.
Inhibition:10 μg/mL anti-CD146 monoclonal antibody AA98 was added in the medium for inhibition.
Target
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(Creative Biolabs Cat# HPAB-0031-YJ, RRID: AB_3111345)
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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| CAT | Product Name | Application | Type |
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| TAB-441MZ-S(P) | Mouse Anti-MCAM Recombinant Antibody; scFv Fragment (TAB-441MZ-S(P)) | FC, WB, IHC | Mouse scFv |
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| CAT | Product Name | Application | Type |
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| TAB-442MZ | Human Anti-MCAM Recombinant Antibody (TAB-442MZ) | FC, WB, IHC | Chimeric (Mouse/Human) IgG1 |
| TAB-442MZ-S(P) | Human Anti-MCAM Recombinant Antibody; scFv Fragment (TAB-442MZ-S(P)) | FC, WB, IHC | Human scFv |
| TAB-443MZ-S(P) | Human Anti-MCAM Recombinant Antibody; scFv Fragment (TAB-443MZ-S(P)) | FC, WB, IHC | Human scFv |
| TAB-442MZ-F(E) | Human Anti-MCAM Recombinant Antibody; Fab Fragment (TAB-442MZ-F(E)) | FC, WB, IHC | Chimeric (Mouse/Human) Fab |
| TAB-443MZ-F(E) | Human Anti-MCAM Recombinant Antibody; Fab Fragment (TAB-443MZ-F(E)) | FC, WB, IHC | Chimeric (Mouse/Human) Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-444MZ | Anti-Human MCAM Recombinant Antibody (TAB-444MZ) | ELISA | Humanized antibody |
| TAB-444MZ-S(P) | Anti-Human MCAM Recombinant Antibody scFv Fragment (TAB-444MZ-S(P)) | ELISA | Humanized antibody |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| HPAB-0493LY-S(P) | Human Anti-MCAM Recombinant Antibody (clone H5L0); scfv Fragment | ELISA | Humanized scfv |
| HPAB-0494LY-S(P) | Human Anti-MCAM Recombinant Antibody (clone H10L2); scfv Fragment | ELISA | Humanized scfv |
| HPAB-0495LY-S(P) | Human Anti-MCAM Recombinant Antibody (clone H14L0); scfv Fragment | ELISA | Humanized scfv |
| HPAB-0496LY-S(P) | Human Anti-MCAM Recombinant Antibody (clone H11L3); scfv Fragment | ELISA | Humanized scfv |
| HPAB-0497LY-S(P) | Human Anti-MCAM Recombinant Antibody (clone H11L4); scfv Fragment | ELISA | Humanized scfv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0493LY-F(E) | Human Anti-MCAM Recombinant Antibody (clone H5L0); Fab Fragment | ELISA | Humanized Fab |
| HPAB-0494LY-F(E) | Human Anti-MCAM Recombinant Antibody (clone H10L2); Fab Fragment | ELISA | Humanized Fab |
| HPAB-0495LY-F(E) | Human Anti-MCAM Recombinant Antibody (clone H14L0); Fab Fragment | ELISA | Humanized Fab |
| HPAB-0496LY-F(E) | Human Anti-MCAM Recombinant Antibody (clone H11L3); Fab Fragment | ELISA | Humanized Fab |
| HPAB-0497LY-F(E) | Human Anti-MCAM Recombinant Antibody (clone H11L4); Fab Fragment | ELISA | Humanized Fab |
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| CAT | Product Name | Application | Type |
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